Flagged RNA re-processing wizard
Description (last modified by )
The wizard for selecting RNA to be processed into mRNA (#424) has the ability to flag some RNA items as not usable. Eg. if there is not enough RNA available (at least 1.1µg is needed). The wizard flags such RNA with
Flag annotations and also put them in the 'Flagged RNA' list.
This wizard should start with the items in the 'Flagged RNA' list and help the user to decide what should happen. In this step there are basically 3 outcomes:
- There is lysate left which can be used to extract additional RNA. If the existing RNA has at least 0.5µg the additional extraction could possibly be pooled with the existing so that the 1.1µg limit is reached.
- There is no lysate, but a backup piece is available in the freezer. If this piece is big enough it can maybe be partitioned again for a second try.
- There is no lysate and no backup.
The flagged RNA might be moved to another list depending on which category it falls into. From there it should be possible for the re-processing to use almost the same workflow as for unprocessed items. This is described in other tickets (to be created).
It is important that re-processed items are tracked at all steps so that it is possible to make special decisions when needed. For example:
- Once we have the re-processed RNA we need to make a decision if it should be pooled with the original RNA or not.
- Plate locations may follow different rules for the re-processed items. Eg. the second batch of RNA/DNA/FlowThrough from a lysate will get different coordinates.