| 1 | = Updating to Reggie 4.3 = |
| 2 | |
| 3 | == 1. Before updating! == |
| 4 | |
| 5 | '''Do not update if there are any mRNA or !NeoPrep library plates in progress. The new implementation of the dilution calculations (#875) may cause incorrect results in the lab protocols for mRNA/Library plates created with Reggie 4.2. |
| 6 | ''' |
| 7 | |
| 8 | == 2. After updating == |
| 9 | |
| 10 | |
| 11 | * Set the `LibPrepTarget` annotation to either `Manual` or `NeoPrep` for all normalization protocols. Also make sure that the `RNATargetAmount` and `RNATargetVolume` is set for the protocols. `RNAMinimalAmount` is optional. |
| 12 | * Check the project default items and make sure that one protocol of each type (Manual/!NeoPrep) is included as a default item. These protocols are used by default by the "Library preparation design" wizards. |
| 13 | |