Soft and hard target amount for NeoPrep
|Reported by:||Nicklas Nordborg||Owned by:||Nicklas Nordborg|
Description (last modified by )
The NeoPrep has a rather wide working range (25-100 ng) of input RNA. We have currently optimized our wizards for the higher amount.
While this works fine for the majority of cases there are a few that it doesn't work for:
- We get a negative dilution volume if the remaining quantity of the RNA is higher than the target amount but the concentration is too low (<8ng/µl since we set target amount to 120ng) so we don't reach this when only taking 15µl.
- We get NotEnoughRemainingQuanity flag when the remaining quantity is lower than the target amount.
Both cases should be solved by calculating how much RNA we get if we take 15µl. If this is higher than the lower input limit accepted by the NeoPrep we should include the RNA and not flag it and set the volume of water to 0.
The currently specified amount that is set as the
RNATargetAmount on pre-normalization protocols is the 'soft' target amount. We need a new annotation for setting the 'hard' target amount.
This change affects several wizards:
- The pre-normalization wizard
- The library preparation design wizards
- The lab protocols for NeoPrep library preparation