wiki:net.sf.basedb.illumina

Illumina plug-ins package for BASE

This package contains plug-ins for the Illumina platform. The plug-ins require that you have data in the form of either:

  • For expression experiments: Illumina Bead Summary (IBS) and Illumina Sentrix® Array binary manifest (BGX) files.
  • For SNP experiments: Illumina SNP manifest files in CSV format, Illumina SNP raw data files from BeadStudio.

Expression data plug-ins in this package:

  • Background correction: Illumina BeadStudio like background correction methods
  • Bead summary importer: Import Illumina Bead Summary files as raw bioassay data
  • BGX feature importer: Create array designs from BGX files
  • BGX reporter importer: Import reporter annotations from BGX files
  • Detection P-value calculation (doc): Illumina BeadStudio detection P-value calculations
  • Overview plots showing 'Control summary' plots.

SNP data plugins in this package:

  • SNP reporter importer: Import reporter annotations from SNP manifest files
  • SNP raw data importer: Creates raw bioassays from SNP raw data files
  • SNP intensity calculator: Extracts data from the raw data files and stores it as a root bioassayset in BASE

Documentation

INSTALL describes how to install the plug-in package. It is important that you read this document. The plug-ins require additional raw data tables and reporter annotation columns in the database before they can be used.

README contains general information about the plug-ins and specific information for expressions experiments.

README_PluginDetails contains detailed information on some of the plug-ins provided by this package.

README_SNP contains specific information for SNP experiments.

README_beadstudio2ibs.txt contains information on how to convert Illumina BeadStudio expression projects to a format usable with this package.

License

GNU General Public License version 3.

Download

Version Revision Date Binary (tar.gz) BASE Version*
1.7 1447 November 2, 2011 illumina-plugins-1.7.tar.gz (135kB) 3.0 changelog
1.6 1266 November 3, 2010 illumina-plugins-1.6.tar.gz (129kB) 2.16 changelog
1.5.1 1234 August 25, 2010 illumina-plugins-1.5.1.tar.gz (129kB) 2.15 changelog
1.5 1222 April 20, 2010 illumina-plugins-1.5.tar.gz (129kB) 2.15 changelog
1.4 1173 Sep 29, 2009 illumina-plugins-1.4.tar.gz (124kB) 2.12 changelog
1.3 969 Feb 25, 2009 illumina-plugins-1.3.tar.gz (102kB) 2.9 changelog
1.2 912 Dec 10, 2008 illumina-plugins-1.2.tar.gz (95kB) 2.9 changelog
1.1 702 May 23, 2008 illumina-plugins-1.1.tar.gz (95kB) 2.6 changelog
1.0 632 March 13, 2008 illumina-plugins-1.0.tar.gz (80kB) 2.6 changelog

* In general, each release works with the specified and later BASE versions, except that releases for BASE 2 do not work with BASE 3.

Source code can be downloaded from the subversion repository. Use the following command to get source code for the 1.7 version:

svn co https://baseplugins.thep.lu.se/svn/plugins/base2/net.sf.basedb.illumina/tags/1.7 illumina-1.7

The latest development (maybe unstable; do not use on a production server) revision can be checked out from the repository using the following command:

svn co https://baseplugins.thep.lu.se/svn/plugins/base2/net.sf.basedb.illumina/trunk illumina-dev

or browse the svn repository.

Contribute

After making modifications to the source code, compose a clear and concise log message to describe those changes and the reasons for them. Then, send an email to the developers list containing your log message and the output of svn diff (from the top of your subversion working copy). If the community members consider your changes acceptable, someone who has commit privileges will add your changes to the public source code tree. Recall that permission to directly commit changes to the repository is granted on merit - if you demonstrate comprehension of subversion, programming competency, and a team spirit, you will likely be awarded that permission.

Feedback

Please use the BASE mailing lists for discussions about the Illumina plug-ins. You have to login to this trac environment to be able to report bugs, enhancement ideas, and feature requests. Login with userid base and password base and use the ticket reporting system.

TODO

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#463
Illumina expression background correction (version 1.7) does not ask for required permissions to write file

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Last modified 4 years ago Last modified on Jun 9, 2021, 7:21:24 PM

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