Opened 10 years ago

Closed 10 years ago

Last modified 10 years ago

#779 closed defect (fixed)

Librararies with very high molarity gives "Low molarity" warning in pooling wizard

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: major Milestone: Reggie v3.4
Component: net.sf.basedb.reggie Keywords:
Cc:

Description (last modified by Nicklas Nordborg)

The current theory is that the high molarity results in such a small volume that when it is rounded to one decimal it gives 0.0 as the result. The subsequent calculations then overflows to a NaN value due to a division by 0. See attached image.

A possible workaround is to increase the pool volume/lib but since it has an upper limit at 20µl it may not be enough in all cases.

Attachments (1)

low-molarity.png (6.7 KB ) - added by Nicklas Nordborg 10 years ago.

Download all attachments as: .zip

Change History (3)

by Nicklas Nordborg, 10 years ago

Attachment: low-molarity.png added

comment:1 by Nicklas Nordborg, 10 years ago

Resolution: fixed
Status: newclosed

(In [3314]) Fixes #779: Librararies with very high molarity gives "Low molarity" warning in pooling wizard

Changed the code so that the rounding is not done until after the mix factor has been calculated.

comment:2 by Nicklas Nordborg, 10 years ago

Description: modified (diff)
Note: See TracTickets for help on using tickets.