Opened 9 years ago

Last modified 3 weeks ago

#752 new enhancement

Schedule new RNAQC from Flagged RNA wizard

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: major Milestone: Reggie v5.x
Component: net.sf.basedb.reggie Keywords:


RNA items may be flagged due to low RQS/RIN value. It would be useful to be able to schedule a new RNAQC measurement from the Flagged RNA wizard.

RNA in this state typically have AutoProcessing=Disable and Flag=LowQualityScore. The flag should not be changed/cleared until we have a new RIN value (assuming that new measurements are done on the Bioanalyzer). The AutoProcessing annotation can't be set to ReProcess since it would push the RNA into library preparation.

I think we need a new value for AutoProcessing that pushes the RNA into the RNAQC path. But it should not mix up with RNA coming from the regular flow since we need to fetch the RNA from the freezer (see #744).

A possible solution is to have two lists on the 'Create aliquots on Bioanalyzer/Caliper plates' much like the new partition wizard (see #740).

Once the new RNAQC items has been created the AutoProcessing annotation on the RNA item need to be reset to Disable. When registering the new values with the 'Register RIN scores for BioAnalyzer plates' there is a possibility to remove the AutoProcessing and flag annotation from the RNA which will cause it to enter the default flow again. Eg. the auto-select feature in the 'Create new mRNA plate' would select it based on the new RIN value.

Change History (2)

comment:1 by Nicklas Nordborg, 9 years ago

Milestone: Reggie v3.xReggie v4.x

Milestone renamed

comment:2 by Nicklas Nordborg, 3 weeks ago

Milestone: Reggie v4.xReggie v5.x

Milestone renamed

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