Opened 10 years ago

Closed 10 years ago

Last modified 9 years ago

#637 closed task (fixed)

Add annotations for lot numbers in the DNA/RNA/FlowThrough registration

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: major Milestone: Reggie v2.16
Component: net.sf.basedb.reggie Keywords:
Cc:

Description (last modified by Nicklas Nordborg)

We would like to keep track of the lot numbers for some reagents used in the lysis and extraction step.

Once a list of annotations has been defined the following changes should be made:

  • The lab protocol should include fields for writing down the lot numbers.
  • The second step of the "DNA/RNA/FlowThrough registration" should have input fields for the annotations.
  • In the last step it should be possible to set different values for some of the annotations per sample.

Lot numbers used in the lysis step are saved as annotations on the Lysate item. Lot numbers used in the extraction step are saved as annotation on the DNA, RNA and FlowThrough items.

List of annotations for lysate:

  • RLT Plus buffer -> RLT_plus
  • Beta-Mercaptoetanol -> BetaMerca
  • Reagent-DX -> !ReDX
  • QIAshredder column -> !Qiashredder

List of annotation for RNA/DNA/FlowThrough

  • AllPrep DNA/RNA Mini kit - AllprepMiniKit
  • ETOH 70% -> ETOH70
  • Buffer RW1 -> RW1
  • Buffer RPE -> RPE
  • Buffer AW1 -> AW1
  • Buffer AW2 -> AW2
  • Buffer EB -> EB
  • RNase free water -> RNaseFreeW
  • AllPrep DNA Mini Spin Column -> AllPrepDNA_col
  • RNeasy Mini Spin Column -> RNeasy_col

Change History (7)

comment:1 by Nicklas Nordborg, 10 years ago

Description: modified (diff)

comment:2 by Nicklas Nordborg, 10 years ago

Milestone: Reggie v2.xReggie v2.16
Status: newassigned

comment:3 by Nicklas Nordborg, 10 years ago

(In [2687]) References #637: Add annotations for lot numbers in the DNA/RNA/FlowThrough registration

Re-designed the lab protocol to include fields for all reagents.

comment:4 by Nicklas Nordborg, 10 years ago

Resolution: fixed
Status: assignedclosed

(In [2690]) Fixes #637: Add annotations for lot numbers in the DNA/RNA/FlowThrough registration

14 new annotations; 4 on Lysate items and 10 on RNA/DNA/FlowThrough items.

The second step in the DNA/RNA/FlowThrough registration wizard contain input fields (optional) for all annotations. The third step allows the annotations to be changed for specific samples via the "Edit" dialog that also is used to modify volumes and add comments.

comment:5 by Nicklas Nordborg, 9 years ago

(In [2717]) References #637: Add annotations for lot numbers in the DNA/RNA/FlowThrough registration

Better balance between header and value columns trying to prevent linebreaks if the margin is large when printing.

comment:6 by Nicklas Nordborg, 9 years ago

(In [2718]) References #637: Add annotations for lot numbers in the DNA/RNA/FlowThrough registration

Better balance between header and value columns trying to prevent linebreaks if the margin is large when printing.

comment:7 by Nicklas Nordborg, 9 years ago

(In [2719]) References #637: Add annotations for lot numbers in the DNA/RNA/FlowThrough registration

Reverting change from [2717] since it was checked in in the wrong branch (trunk). [2718] is the same change in the correct branch (2.16-stable)

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