Opened 5 months ago

Closed 5 months ago

#1554 closed enhancement (fixed)

Generate counts and plots for SBS signatures

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: major Milestone: Reggie v4.51
Component: net.sf.basedb.reggie Keywords:
Cc:

Description

The SBS mutational signatures are describer here: https://cancer.sanger.ac.uk/signatures/sbs/

We should use the information that was added to the VCF file by #1552 to count the number of mutations and output a text file with the counts for the 96 SBS contexts. This text file can then be plotted in a nice pdf by the SigProfilerPlotting package: https://github.com/AlexandrovLab/SigProfilerPlotting

Change History (9)

comment:1 by Nicklas Nordborg, 5 months ago

In 7488:

References #1554: Generate counts and plots for SBS signatures

Updated the container definition to also include the SigProfilerPlotting package. Increased the version number.

comment:2 by Nicklas Nordborg, 5 months ago

In 7489:

References #1554: Generate counts and plots for SBS signatures

Added a python script that creates a text file SBS_96.txt with counts and a PDF with the plot.

comment:3 by Nicklas Nordborg, 5 months ago

In 7490:

References #1554: Generate counts and plots for SBS signatures

Fixed a bug were the REF was used instead of the left base causing counts to be assigned to incorrect SBS categories.

comment:4 by Nicklas Nordborg, 5 months ago

In 7491:

References #1554: Generate counts and plots for SBS signatures

Changed iteration order of the SBS categories so that mutations of the same type are next to each other.

comment:5 by Nicklas Nordborg, 5 months ago

In 7492:

References #1554: Generate counts and plots for SBS signatures

Added thumbnails of the PDF plot to the raw bioassay list and view pages.

comment:6 by Nicklas Nordborg, 5 months ago

In 7493:

References #1554: Generate counts and plots for SBS signatures

Added a link to the PDF plot in the case summary.

comment:7 by Nicklas Nordborg, 5 months ago

In 7494:

References #1554: Generate counts and plots for SBS signatures

Added a thumbnail of the SBS plot to the confirmation wizard.

comment:8 by Nicklas Nordborg, 5 months ago

In 7496:

References #1543 and #1554. Include the SBS counts and PDF plot in the release export.

comment:9 by Nicklas Nordborg, 5 months ago

Resolution: fixed
Status: newclosed
Note: See TracTickets for help on using tickets.