#1552 closed enhancement (fixed)

Include the ReferenceBases annotation when running Mutect2

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: major Milestone: Reggie v4.51
Component: net.sf.basedb.reggie Keywords:
Cc:

Description

This is a configuration change only. We should add --annotation ReferenceBases to the !Mutect2Options setting in reggie-config.xml.

This will add an INFO-level annotation REF_BASES to the VCF file that contain 21 bases. 10 bases before and after the REF with the REF in the middle.

From this annotation we can modified the vcfanno configuration to extract the middle 3 bases and store it as a separate annotation. This information is already in the RNAseq variant calling with name TRINUCLEO so we will use the same.

Change History (2)

comment:1 by Nicklas Nordborg, 12 months ago

In 7481:

References #1552: Include the ReferenceBases annotation when running Mutect2

Added --annotation ReferenceBases to reggie-config.xml.

comment:2 by Nicklas Nordborg, 12 months ago

Resolution: fixed
Status: newclosed
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