| 1 | = Updating to Reggie 4.23 = |
| 2 | |
| 3 | TODO - when to do the different steps! |
| 4 | |
| 5 | TODO - state requirements: no current sequencing or secondary analysis jobs |
| 6 | |
| 7 | == 1. Set value for Pipeline annotation on existing items == |
| 8 | |
| 9 | Almost all existing items from Library and downstream must be given a value for the **Pipeline** annotation. The table below specifies the value |
| 10 | to set for the Pipeline annotation for different types of items. It should be possible to do a manual batch export/import for each row or groups of rows. |
| 11 | |
| 12 | || **Item type** || **Subtypes** || **Additional filter** || **Pipeline value** || |
| 13 | || Extract || Library, !PooledLibrary, !PooledLibraryAliquot || - || RNAseq || |
| 14 | || Physical bioassay || !FlowCell || - || RNAseq || |
| 15 | || Derived bioassay || !SequencingRun, !DemuxedSequences, !MergedSequences || - || RNAseq || |
| 16 | || Derived bioassay || !MaskedSequences, !AlignedSequences || Job type = !HisatAlign || TODO || |
| 17 | || Derived bioassay || !MaskedSequences, !AlignedSequences || Job type <> !HisatAlign || Legacy || |
| 18 | || Raw bioassay || Cufflinks || - || Legacy || |
| 19 | || Raw bioassay || !StringTie || - || TODO || |
| 20 | || Protocol || Demuxing || - || RNAseq || |
| 21 | || Software || Demuxing || - || RNAseq || |
| 22 | |
| 23 | == 2. Other updates to existing items == |
| 24 | |
| 25 | Existing Hardware items of type=Sequencer should be updated with a value for the **!FlowCellType** annotation. It should be either !NextSeq or !HiSeq. |
| 26 | |
| 27 | == 3. New items that should be created == |
| 28 | |
| 29 | || **Item type** || **Subtypes** || **Additional settings** || **Comment** || |
| 30 | || Protocol || DNANormalization || !TargetAmount, !TargetMinimalAmount, !TargetVolume, MIPS_Pool || The protocol to use for normalizing DNA that should go into the MIPs pipeline. || |
| 31 | || Protocol || Library preparation || !LibPrepTarget=MIPs || One or more protocols that will appear in the "MIPs library registration" wizard || |
| 32 | || Protocol || Pooling || !LibPrepTarget=MIPs || One or more protocols that will appear in the MIPs pooling wizard || |
| 33 | || Protocol || Demuxing || Pipeline=MIPs || One or more protocols that will appear in the MIPs demux wizard || |
| 34 | || Software || Demuxing || Pipeline=MIPs || One or more software entries that will appear in the MIPs demux wizard || |
| 35 | |
| 36 | |