Version 1 (modified by 5 years ago) ( diff ) | ,
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Updating to Reggie 4.23
TODO - when to do the different steps!
TODO - state requirements: no current sequencing or secondary analysis jobs
1. Set value for Pipeline annotation on existing items
Almost all existing items from Library and downstream must be given a value for the Pipeline annotation. The table below specifies the value to set for the Pipeline annotation for different types of items. It should be possible to do a manual batch export/import for each row or groups of rows.
Item type | Subtypes | Additional filter | Pipeline value |
Extract | Library, PooledLibrary, PooledLibraryAliquot | - | RNAseq |
Physical bioassay | FlowCell | - | RNAseq |
Derived bioassay | SequencingRun, DemuxedSequences, MergedSequences | - | RNAseq |
Derived bioassay | MaskedSequences, AlignedSequences | Job type = HisatAlign | TODO |
Derived bioassay | MaskedSequences, AlignedSequences | Job type <> HisatAlign | Legacy |
Raw bioassay | Cufflinks | - | Legacy |
Raw bioassay | StringTie | - | TODO |
Protocol | Demuxing | - | RNAseq |
Software | Demuxing | - | RNAseq |
2. Other updates to existing items
Existing Hardware items of type=Sequencer should be updated with a value for the FlowCellType annotation. It should be either NextSeq or HiSeq.
3. New items that should be created
Item type | Subtypes | Additional settings | Comment |
Protocol | DNANormalization | TargetAmount, TargetMinimalAmount, TargetVolume, MIPS_Pool | The protocol to use for normalizing DNA that should go into the MIPs pipeline. |
Protocol | Library preparation | LibPrepTarget=MIPs | One or more protocols that will appear in the "MIPs library registration" wizard |
Protocol | Pooling | LibPrepTarget=MIPs | One or more protocols that will appear in the MIPs pooling wizard |
Protocol | Demuxing | Pipeline=MIPs | One or more protocols that will appear in the MIPs demux wizard |
Software | Demuxing | Pipeline=MIPs | One or more software entries that will appear in the MIPs demux wizard |
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