| 1 | = Updating to Reggie 4.15 = |
| 2 | |
| 3 | === New !StringTie pipeline in the Hisat secondary analysis === |
| 4 | |
| 5 | In `reggie-config.xml` the following changes are required: |
| 6 | |
| 7 | * In the `<programs>` section, a `<stringtie>` sub-section should be added with the full `<path>` to the !StringTie executable. |
| 8 | * A `<stringtie>` section with !StringTie configuration parameters should be added. The configuration file shipped with Reggie should contain functional default values. |
| 9 | |
| 10 | === New annotation on feature extraction software and protocols === |
| 11 | |
| 12 | A new annotation type, `ExpressionType` has been added. Find existing software and protocols with `Type=Feature extraction` and set the `ExpressionType` annotation to `Cufflinks`. |
| 13 | |
| 14 | Create a new software and protocol item for the !StringTie pipeline. They should have `Type=Feature extraction` and `ExpressionType=StringTie`. Keep the `Add as project default` checkbox checked and the `Replace existing default` unchecked. |