Opened 8 years ago
Closed 8 years ago
#773 closed enhancement (fixed)
The mRNA wizard must be smarter when auto-selecting pre-normalized RNA
Reported by: | Nicklas Nordborg | Owned by: | Nicklas Nordborg |
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Priority: | critical | Milestone: | Reggie v3.4 |
Component: | net.sf.basedb.reggie | Keywords: | |
Cc: |
Description (last modified by )
If the 'Pre-normalized RNA' option is selected in the auto-select function, it will select all pre-normalized RNA not accounting for the intended protocol (see #762).
This means that RNA which is pre-normalized for the NeoProp will be flagged if the mRNA wizard is used to create a manual TruSeq plate. Other mismatches may create incorrect mixing protocols.
Seems like the mRNA wizard will allow all pre-normalized RNA to be included but with a warning if the quantity is too low. The lab protocol doesn't make any checks either but will show incorrect volumes.
The mRNA wizard need to be aware of the different library preparation protocols and only auto-select RNA that is for the intended use. Exactly how to do this needs to be investigated.
Change History (7)
comment:1 Changed 8 years ago by
Summary: | The auto-select function in the mRNA wizard must be smarter when selecting pre-normalized RNA → The mRNA wizard must be smarter when auto-selecting pre-normalized RNA |
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comment:2 Changed 8 years ago by
Description: | modified (diff) |
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comment:3 Changed 8 years ago by
Description: | modified (diff) |
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comment:4 Changed 8 years ago by
Status: | new → assigned |
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comment:5 Changed 8 years ago by
comment:6 Changed 8 years ago by
comment:7 Changed 8 years ago by
Resolution: | → fixed |
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Status: | assigned → closed |
(In [3270]) References #773: The mRNA wizard must be smarter when auto-selecting pre-normalized RNA
Added selection of normalization protocol in the auto-select RNA function so that it only selects RNA that has been pre-normalized with the given protocol.