Changes between Initial Version and Version 2 of Ticket #762


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Timestamp:
Apr 21, 2015, 3:00:19 PM (10 years ago)
Author:
Nicklas Nordborg
Comment:

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  • Ticket #762

    • Property Milestone Reggie v3.x → Reggie v3.4
    • Property Summary Pre-normalization changes due to neoprep → Pre-normalization changes due to NeoPrep

  • Ticket #762 – Description

    initial v2  
    1 The neoprep require only 100ng of input rna in ~25µl (there is a range but I don't remember the limits). The current pre-normalization is hard-wired for the manual protocol which is optimized for 1.1µg in 50µl.
     1The neoprep require only 100ng of input rna in 25-45µl. The current pre-normalization is hard-wired for the manual protocol which is optimized for 1.1µg in 50µl.
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    3 The pre-normalization need to be modified so that it also works with the neoprep. The required RNA quantity and volume should be selectable (a few fixed options??)
     3The pre-normalization need to be modified so that it also works with the !NeoPrep. The required RNA quantity and volume should be selectable, with at least the following presets:
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    5 Due to the much lower quantity of RNA the pre-normalization will need to support mixing a larger volume (to avoid volumes less than 1µl). This also means that the mRNA wizard need to be aware of this, since it currently assumes that everything is used from the pre-normalized aliquot.
     5 * The old (dUTP) method (1.1µg in 50µl)
     6 * !TruSeq manual method (0.5µg in 45µl)
     7 * !NeoPrep method (0.1µg in 45µl)
     8
     9Due to the much lower quantity of RNA for !NeoPrep the pre-normalization will need to support mixing a larger volume (to avoid volumes less than 1µl). This also means that the mRNA wizard need to be aware of this, since it currently assumes that everything is used from the pre-normalized aliquot.