Opened 10 years ago
Last modified 10 years ago
#762 closed task
Pre-normalization changes due to neoprep — at Initial Version
Reported by: | Nicklas Nordborg | Owned by: | Nicklas Nordborg |
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Priority: | major | Milestone: | Reggie v3.4 |
Component: | net.sf.basedb.reggie | Keywords: | |
Cc: |
Description
The neoprep require only 100ng of input rna in ~25µl (there is a range but I don't remember the limits). The current pre-normalization is hard-wired for the manual protocol which is optimized for 1.1µg in 50µl.
The pre-normalization need to be modified so that it also works with the neoprep. The required RNA quantity and volume should be selectable (a few fixed options??)
Due to the much lower quantity of RNA the pre-normalization will need to support mixing a larger volume (to avoid volumes less than 1µl). This also means that the mRNA wizard need to be aware of this, since it currently assumes that everything is used from the pre-normalized aliquot.