Opened 10 years ago

Closed 10 years ago

#572 closed enhancement (fixed)

Use different barcode for pools from the same plate

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: major Milestone: Reggie v2.15
Component: net.sf.basedb.reggie Keywords:


A pool typically consist of 21 samples of the 24 that make up 3 columns on a library plate. The bottom row is typically used for quality control. With the current design for selecting barcodes it is always AD008, AD016 and AD027 that are in the bottom row and the same 21 barcodes are used in each of the 4 pools. While there is nothing wrong with this, it makes it possible for mistakes to pass unnoticed when clustering flow cells. Each of the 4 pools should be put in 4 different lanes on 2 flow cells. If there is a mixup in this step (for example switching 2 lanes) it is not possible to detect it.

The idea is to change the barcode layout so that even if the bottom row is not included in the pools each pool get a different set of 21 barcodes. Then, if an unexpected barcode is showing up in a lane where it shouldn't be this is an indication that a mistake was made when clustering.

A simple change to the barcode layout is to use the following schema:

  • Pool i: Same as today
  • Pool ii: First column is swapped (eg. AD008 at top and AD001 at bottom)
  • Pool iii: First and second column is swapped.
  • Pool iv: All columns are swapped

To avoid mistakes when adding barcodes to the libraries it is important that the swapped columns are easy notice!

Change History (2)

comment:1 by Nicklas Nordborg, 10 years ago

(In [2218]) References #572: Use different barcode for pools from the same plate

Added a new pool schema (A-B-C-D) to the 4x24 pool layout and changed the name of the old (FULL) to (A-A-A-A). The new schema is the default.

Hopefully the colors of the new pool combinations trickle down to all subsequent wizards.

comment:2 by Nicklas Nordborg, 10 years ago

Resolution: fixed
Status: newclosed
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