Specify amount to use from *.dil items in pool
|Reported by:||Nicklas Nordborg||Owned by:||Nicklas Nordborg|
When dynamic mixing strategy is used for a pool, libraries which need less than 1µl in the pool are mixed separately before they are added to the pool. The current strategy is to mix the library to 2nM and then add the specified 'Average volume' (5µl) to the pool.
In most cases this is good enough, but if there are also many libraries with low concentration it may happen that we end up with a negative EB volume to add in the end. In this case it may be useful to mix less EB with the high-concentration libraries then add less to the pool (5nM and 2µl or 10nM and 1µl) so that the final EB volume increases (hopefully to a value > 0).