#499 closed task (fixed)
Export sample sheet for flow cell
Reported by: | Nicklas Nordborg | Owned by: | Nicklas Nordborg |
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Priority: | major | Milestone: | Reggie v2.13 |
Component: | net.sf.basedb.reggie | Keywords: | |
Cc: |
Description
The sample sheet is a tab-separated text file with data about the samples that are put into a flow cell, including information about barcoding. The sample sheet is used by analysis software after the sequencing.
Change History (8)
comment:1 by , 11 years ago
Status: | new → assigned |
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comment:2 by , 11 years ago
comment:3 by , 11 years ago
(In [2030]) References #499: Export sample sheet for flow cell
The exporter now uses the ReadString stored as an annotation to the Flow cell instead of asking for it as a job parameter.
comment:4 by , 11 years ago
comment:5 by , 11 years ago
comment:6 by , 11 years ago
comment:7 by , 11 years ago
Resolution: | → fixed |
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Status: | assigned → closed |
comment:8 by , 11 years ago
(In [2105]) References #499: Export sample sheet for flow cell
The ReadString
parameter is now calculated automatically from the SequencingCycles
values. It is assumed that the SequencingCycles
consists of 3 values separated by '-'. The first and last values are normal reads and use 'Y'/'N' and the middle value is index read and use 'I' and 'J'.
Each value is subtracted by one and used for the 'Y' or 'I' part. Then 'N1' or 'J1' is added.
If it is not possible to calculate the ReadString
the default value 'Y54N1I6J1Y53N1' is used.
For this to work, the plug-in configuration can no longer have any parameter for the ReadString
since it has higher priority than the default values.
(In [2021]) References #499: Export sample sheet for flow cell
First implementation of an export plug-in. It is yet a bit uncertain were some information should be coming from. Eg. the ReadString is now configuration parameter for the plug-in, but it may be better to store this as an annotation on the flow cell (as is done for 'operator' and 'flow cell id'). Some information is still hard-coded in the plug-in (eg. concentration = 12pM).