Put RNA aliquots on bioplate to be used by the Caliper machine
|Reported by:||Nicklas Nordborg||Owned by:||Nicklas Nordborg|
The Caliper machine takes 8x12 plates for batch processing 96 extracts at a time. Aliquots are put on the plate at regular intervals, and it will usually take a couple of weeks to fill up a plate.
We need a reggie wizard that is able to pick RNA extracts that has not yet been processed and put aliquots on the current plate or on a new plate. New plates should automatically be generated with a naming convention and a running number.
Each use of the wizard should generate a "bioplate event" with protocol information, dates, etc. Used quantity can be different for each processed RNA extract and there may also be other values that need to be entered for each aliquot.
It is also possible that external RNA is used on the plate. Since we don't have any information about that in the BASE installation the wizard must allow "empty" wells on the plate.
The general outline of the wizard is:
- Show a list of unprocessed RNA and allow the user to select which one to process in this event. An advanced mode should also allow the user to select already processed RNA since the procedure may have to be repeated a second or third time.
- Let the user select an existing plate or create a new plate to use in this event.
- For each selected RNA, allow the user to select position and other values. There should be a function that can suggest default positions, but it must be possible to override by the user.
- Save everything to BASE.