Opened 2 years ago
Closed 2 years ago
#1426 closed defect (fixed)
Warn/fix unreasonble values for RNA remaining quantity in JSON files
Reported by: | Nicklas Nordborg | Owned by: | Nicklas Nordborg |
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Priority: | major | Milestone: | Reggie v4.41.1 |
Component: | net.sf.basedb.reggie | Keywords: | |
Cc: |
Description
The JSON files that are imported by the "Import externally processed samples" wizard seem to have unreasonable value for the original and remaining quantity. It seems like only a few nanograms have been used in the library prep. The higher the concentration it seems like a lot less has been used. The values makes more sense if the difference between original and remaining quantity is interpreted as a volume (in µl).
Example, in the JSON we have:
- Original quantity (ng):
23980
- Remaining quantity (ng):
23978
- Concentration (ng/ul):
436
It is not reasonable that only 2ng was used, but 2µl*436ng/µl=872ng is more likely (though rounding the quantities to whole numbers probably affect the result).
The import wizard should check the original and remaining quantity and issue a warning if the difference is less than 100ng. It should also try to re-calculate the quantities based on the assumption that the difference is a volume in µl and if it makes more sense use that when importing instead.
Change History (2)
comment:1 by , 2 years ago
comment:2 by , 2 years ago
Resolution: | → fixed |
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Status: | new → closed |
In 6943: