#1015 closed enhancement (fixed)
Add masking step to Hisat pipeline
Reported by: | Nicklas Nordborg | Owned by: | Nicklas Nordborg |
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Priority: | major | Milestone: | Reggie v4.15 |
Component: | net.sf.basedb.reggie | Keywords: | |
Cc: |
Description
The same masking that is done in the legacy pipeline before Tophat is run should also be done in the Hisat pipeline.
This means that we need to re-introduce the MaskedSequences item. Statistics should be imported to the PM_READS annotation.
Change History (5)
comment:1 by , 7 years ago
Status: | new → assigned |
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Summary: | Add masing step to Hisat pipeline → Add masking step to Hisat pipeline |
comment:2 by , 7 years ago
comment:3 by , 7 years ago
Resolution: | → fixed |
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Status: | assigned → closed |
comment:4 by , 7 years ago
comment:5 by , 7 years ago
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(In [4656]) References #1015: Add masking step to Hisat pipeline
The masking is step is now included in the job script.
Software and protocol parameters are selected from the project default (NOT using the Tophat vs. Hisat setting since we use the same in both pipelines).
A
MaskedSequences
item is created and thePM_READS
annotions is populated with information from the mask step.There are still some places in the code that may be "suprised" by the masked parent item (causing a NPE). This needs to be fixed.
The Hisat confirmation wizard should also display the PM_READS annotation.