Imports into BASE 2, raw data files and experimental metadata from a zipped archive containing a tab2mage formatted description of the experiment and a set of raw data files.
The Tab2MageImporter plugin batch loads raw data files and inserts all experimental descriptors in BASE2 by parsing a tab2mage file and raw data files from a zipped archive. The tab2mage specifications is provided by the Microarray Informatics Team at the EBI, at the EBI, Cambridge and are available from http://tab2mage.sourceforge.net/.
The Tab2MageImporter plugin is started from the Experiment Item View Page. This means that the user needs to create an experiment first and then execute the plugin from this page by clicking on the Import tab.
Please read the next section to ensure the BASE environment has been properly set up before trying to run Tab2MageImporter plugin.
Reporter Importer Plugin - Required for loading Reporters
Reporter Map Importer Plugin – Required for mapping features to Reporters when defining an Array Design
Raw Data Importer Plugin – Required for loading raw data and import values associated to BASE rawBioassays. This is a critical requirement, for multi-channel experiments, before running the Tab2MageImporter. Users must ensure that raw data import plugin has been properly configured and is able to import a raw datafile of the type of the submission.
The definition for the above plug-ins is available by default in Base 2. The plugins should be configured by a Base 2 administrator and shared to everyone or a suitable group of target users.
AnnotationTypeCvImporter Plugin – Required for loading annotation types. The plugin must have a proper configuration defined to import an annotation type file created by the Tab2MageImporter plugin when parsing the tab2mage file.
Please ensure that you have used all the plugins above appropriately, before running Tab2Mage importer. Read the README file in the documentation section for further details.
Click AnnotationTypeCvImporter for further information on Annotation Type and Cv Importer plugin and for other plugins, please read base2 documentation.
Also, do make sure that Array Design(s) corresponding to the one(s) referenced in the Array[accession] field of the tab2mage file have been properly created in BASE2. If not, the plugin will fail. For all non-Affymetrix Array Designs,this means that Reporters have been loaded and Feature to Reporter Map have been attached to Array Designs. Those tasks can be only be achieved if the above plugins have been properly set up and configured.
AffyArrayDesignBatchImporter Plugin .
– for batch uploading affymetrix array designs. Useful to upload all array designs used in an experiment in one go before running the Tab2Mage Importer.
The README files below contains information on how to install and use the plug-in.
|Installation and Use||Known Issues/Features?||Sample Tab2Mage Import Work Flow||Pooling Note|
|Rules for Creating Spreadsheet||Sample Spreadsheets|
|Rules||Affymetrix | Agilent | Genepix|
Bugs will be fixed. Volunteers welcome to help extend the plug-in.
Philippe Rocca-Serra – European Bioinformatics Institute, Cambridge, UK, Net-Project technical coordinator.
We would like to thank:
Charles Girardot <charles.girardot@…> and Sajoscha Sauer <sauer@…> at EMBL, Germany whose tab2mage Base1Loader code ( bloader.jar), is used and adapted as a dependent jar for this tab2mage importer implementation.
Misha Bayer <sbrn@…> at the Plant Bioinformatics Group, Scottish Crop Research Institute for allowing us use some code from his batchimporter for the tab2mage importer.
Last but not least, the BASE team at Lund, keep up the good work guys.
|Version||Plugin jar file||Dependent jars||Change Log|
|2||Tab2MageImporter.jar|| bloader.jar | ebi-plugins-utils.jar | AnnotationTypeCvImporter.jar |
mail.jar | activation.jar
Sample archives: Genepix, Agilent, Affymetrix and GenePix with Pooling