Opened 6 years ago

Closed 5 years ago

#749 closed enhancement (fixed)

Allow extraction of less than 12 samples

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: minor Milestone: Reggie v3.5
Component: net.sf.basedb.reggie Keywords:
Cc:

Description

The current implementation of the DNA/RNA/FlowThrough registration wizard is limited to always process 12 samples at a time.

In the future we probably have to be able to process fewer samples in some cases due to timing considerations. The wizard should be updated to allow fewer samples to be selected.

The major issue is that the QiaCube positions must be generated according to spceific scheme in order to keep it properly balanced. We must also discuss how to generate the positions for the NanoDrop step since those are directly related to the QiaCube position.

Change History (7)

comment:1 Changed 5 years ago by Nicklas Nordborg

Milestone: Reggie v3.xReggie v3.5
Status: newassigned

comment:2 Changed 5 years ago by Nicklas Nordborg

(In [3466]) References #749: Allow extraction of less than 12 samples

The "Lab tracking protocol for Allprep isolation" now has support for selecting 1-12 samples. QIAcube positions are generated using the information from https://www.qiagen.com/ca/resources/download.aspx?id=61a6d41e-2967-4a62-9a11-7367af1b13af&lang=en

1 and 11 samples are handled by adding a counterbalance sample in position 7 or 12.

An image for the current configuration is included in the lab protocol.

An extra column for NanoDrop position is included in the protocol, but positions are currently not generated. It has been decided to fill the NanoDrop plate without "gaps", except that RNA items are re-started in the next column. The final "blank" position should also always be included.

comment:3 Changed 5 years ago by Nicklas Nordborg

(In [3467]) References #749: Allow extraction of less than 12 samples

Calculate NanoDrop? positions in the lab protocol.

comment:4 Changed 5 years ago by Nicklas Nordborg

(In [3468]) References #749: Allow extraction of less than 12 samples

The "DNA/RNA/FlowThrough registration" now has support for 1-12 samples. QIAcube positions are genered using the same schema as for the protocol. NanoDrop are calculated by orderingen the samples by QIAcube positions and not leaving any "holes" in the plate. RNA is always started in a new column (number 2 or 3 depending on the number of samples).

comment:5 Changed 5 years ago by Nicklas Nordborg

(In [3469]) References #749: Allow extraction of less than 12 samples

Adding ReservedBy annotation to mark lysates that has been selected for extraction.

The annotation is used to control the default selection. Lysates that are reserved will only be automatically selected when the same user is logged in. Other users will skip to the next unreserved lysates.

The annotation is not blocking and all users may override the default selection.

comment:6 Changed 5 years ago by Nicklas Nordborg

(In [3471]) References #749: Allow extraction of less than 12 samples

Checking number of free wells on RNAQC plates against the number of selected RNA items. It is an error condition to select a plate with too few free wells, a new plate must be created instead.

comment:7 Changed 5 years ago by Nicklas Nordborg

Resolution: fixed
Status: assignedclosed
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