Opened 7 years ago

Closed 7 years ago

Last modified 7 years ago

#457 closed task (fixed)

Generate lab protocol for cDNA -> Library preparation

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: major Milestone: Reggie v2.12
Component: net.sf.basedb.reggie Keywords:
Cc:

Description (last modified by Nicklas Nordborg)

This is the second wizard in the cDNA to Library processing group of wizards (see #423).

The protocol is needed before the actual lab work is started. The lab protocol should include the following information:

  • Name of library
  • Work plate position
  • Name of TruSeq? adaptor

Change History (12)

comment:1 Changed 7 years ago by Nicklas Nordborg

Summary: Generate lab protocol and other files needed for cDNA -> Library preparationGenerate lab protocol for cDNA -> Library preparation

comment:2 Changed 7 years ago by Nicklas Nordborg

Status: newassigned

comment:3 Changed 7 years ago by Nicklas Nordborg

(In [1814]) References #456 and #457. Added support for manually setting barcodes. Check for duplicate barcodes in the same pool. Added list- and plate-view protocols for library preparation.

comment:4 Changed 7 years ago by Nicklas Nordborg

Milestone: Reggie v2.xReggie v2.12

comment:5 Changed 7 years ago by Nicklas Nordborg

(In [1894]) References #457: Generate lab protocol for cDNA -> Library preparation

Use Library bioplates directly instead of cDNA items with non-created child Library items.

comment:6 Changed 7 years ago by Nicklas Nordborg

Description: modified (diff)

comment:7 Changed 7 years ago by Nicklas Nordborg

(In [1917]) References #457: Generate lab protocol for cDNA -> Library preparation

Changed title to include the plate name and protocol type since it gives better file names when printing to PDF.

comment:8 Changed 7 years ago by Nicklas Nordborg

(In [1927]) References #425, #457, #479. Format comment text with line breaks.

comment:9 Changed 7 years ago by Nicklas Nordborg

(In [1929]) References #424, #425, #457, #479. Re-designed protocols to make the header section a bit more compact when the comment text is large. Display a warning when the remaining RNA quanitity is below 1.5 times the required amount (eg. 1.1µg or 1.22µg).

comment:10 Changed 7 years ago by Nicklas Nordborg

(In [1933]) References #457: Generate lab protocol for cDNA -> Library preparation

Do not show tables until all information has been loaded.

comment:11 Changed 7 years ago by Nicklas Nordborg

Resolution: fixed
Status: assignedclosed

comment:12 Changed 7 years ago by Nicklas Nordborg

(In [2001]) References #457: Generate lab protocol for cDNA -> Library preparation

Adding more date and operator fields to the lab protocol.

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