Opened 12 years ago
Last modified 12 years ago
#386 closed task
Qiacube/nanodrop wizard — at Version 1
| Reported by: | Nicklas Nordborg | Owned by: | Nicklas Nordborg |
|---|---|---|---|
| Priority: | critical | Milestone: | Reggie v2.6 |
| Component: | net.sf.basedb.reggie | Keywords: | |
| Cc: |
Description (last modified by )
The Qiacube machine is used with Lysate extracts to create RNA, DNA and FlowThrough items (also extracts). We need a wizard to register information about this process. It needs to keep track of used quantities, bioplate positions, and several annotations:
QiaCubeDateQiaCubeRunNoQiaCubePositionQiaCubeOperator
After the extraction the NanoDrop machine is used to measure the following annotations (on RNA and DNA):
NDConcND260by230ND260by280
The Lysate wizard (#388) is used first to create the lab protocol. This wizard will start by finding the Lysate extracts that was used (eg. they have a date but no child RNA or DNA items). Information about the lysis and Qiacube steps are recorded with protocol, operator, actual date, quantities, etc.
The second step generates a file with sample names that can be imported to the NanoDrop machine to map wells to samples.
The third step create child items (DNA, RNA and FlowThroguh) for the Lysate items. The data file generated by the NanoDrop machine should be used to import the ND* annotations.
