Changes between Version 8 and Version 9 of se.lu.onk.IlluminaSNPNormalization
- Timestamp:
- Jun 9, 2008, 2:20:33 PM (17 years ago)
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se.lu.onk.IlluminaSNPNormalization
v8 v9 20 20 tQN is written in R with a Perl wrapper, so both R and Perl are required. Required Perl modules are: File::Spec, Getopt::Long, IO::File and Pod::Usage (http://www.cpan.org). Required R package is limma (http://www.bioconductor.org). 21 21 22 ''Installation'' 23 Download and unzip the file available under the section ''Download tQN'' on this page. 24 25 OS X or Linux: 26 The programs should run as they are. You need R and perl in your path. 27 28 Windows: 29 Depending on how you have installed R and Perl on your system you may have to edit the variable ''$R_command'' at the beginning of the file ''tQN_normalize_samples.pl'' so it contains the full path to your R executable. For example, we have successfully used tQN using !ActivePerl on a Window system with the following ''$R_command'': 30 {{{ 31 # Mac OS X and Linux 32 # my $R_command="R --vanilla --no-save --slave < tQN.R"; 33 # Windows 34 my $R_windows=File::Spec->canonpath('C:/"Program Files"/R/R-2.7.0/bin/Rscript'); 35 my $R_command="$R_windows --vanilla tQN.R"; 36 }}} 37 38 22 39 ''Input data format'' 23 40 tQN is applied to data exported from !BeadStudio. For a set of samples, the file exported from !BeadStudio should be tab-delimited in the following format: … … 30 47 31 48 {{{ 32 ./split_beadstudio_samples.pl --beadstudio_file=example/example_beadstudio_data.txt49 split_beadstudio_samples.pl --beadstudio_file=example/example_beadstudio_data.txt 33 50 }}} 34 51 where ''example_beadstudio_data.txt'' is a file exported from !BeadStudio in the format described above. … … 41 58 42 59 {{{ 43 ./tQN_normalize_samples.pl --beadchip=humancnv370-duo60 tQN_normalize_samples.pl --beadchip=humancnv370-duo 44 61 }}} 45 62 … … 47 64 48 65 {{{ 49 ./tQN_normalize_samples.pl --beadchip=humancnv370-duo --output_format=PennCNV66 tQN_normalize_samples.pl --beadchip=humancnv370-duo --output_format=PennCNV 50 67 }}} 51 68