Changes between Version 1 and Version 2 of net.sf.basedb.reggie/notes425
- Timestamp:
- Jan 31, 2020, 8:29:57 AM (5 years ago)
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net.sf.basedb.reggie/notes425
v1 v2 1 1 = Updating to Reggie 4.25 = 2 2 3 == A. Before updating Reggie == 3 4 4 == 1. New/updated pipeline scripts == 5 === 1. Disable the Variant calling pipeline === 6 7 Disable auto-confirmation for the variant calling by setting `AutoProcessing=Disable` on 8 the **Variant Calling Pipeline** item list. 9 10 === 2. Install new/updated pipeline scripts === 5 11 6 12 There are new pipeline scripts: http://baseplugins.thep.lu.se/browser/other/pipeline/trunk … … 11 17 * `Rscript.sh` 12 18 13 == 2. New databases for annotating variants==19 === 3. Install new databases for annotating variants === 14 20 15 21 The following databases have been updated: … … 28 34 the databases. 29 35 30 == 3. Create new SCAN-B databases == 36 == B. Update Reggie == 37 38 Update Reggie to version 4.25 as usual. Do not forget to run the Installation wizard. 39 40 == C. After updating Reggie == 41 42 === 1. Create new SCAN-B databases === 31 43 32 44 Use the new Reggie wizard (Build SCAN-B variant database) to create new databases for the existing SCAN-B samples. … … 40 52 * `scanb-normals.vcf.gz` (and a corresponding .tbi file) 41 53 54 === 2. Test the variant calling pipeline === 55 56 The annotation and filtering part of the variant calling pipeline can now be tested. Select some alignments that already have raw variant calls. 57 58 === 3. Enable the Variant calling pipeline === 59 60 Enable auto-confirmation for the variant calling by removing `AutoProcessing=Disable` from 61 the **Variant Calling Pipeline** item list. 62 63 === 4. Complete the annotation and filtering step for all alignments that already have raw variant calling === 42 64 43 65