Version 8 (modified by 11 years ago) ( diff ) | ,
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Updating to Reggie 2.13
The following items should be performed after updating to Reggie 2.13.
1. Flag some libraries and their parent RNA item
Due to a bug (see #508), libraries which got a Qubit concentration measurement of 0 ng/µl have been left in a dead end state. To ensure that new RNA is extracted in the future, a flag must be set manually and the parent RNA items must be added to the Flagged RNA list.
- To find the libraries in question go to the Biomaterial LIMS->Extracts page and filter on Type=Library and CA_Molarity=0.
- Edit each library item and set the annotation: Flag=ExcludedFromPool.
- Find the parent RNA item for each library and set the annotation: Flag=ExcludedFromPool.
- Add all parent RNA items to the Flagged RNA biomaterial list.
- Check that the selection list in the Create pooled libraries no longer include already registered library plates.
2. Fix PoolConc values for all *.dil extracts
Due to a bug (see #507), all *.dil
extracts have been registered with an incorrect value for the PoolConc annotation. This need to be fixed by a manual export/import procedure.
- To find the libraries in question go to the Biomaterial LIMS->Extracts page and filter on name=%.dil.
- To avoid rounding errors in the exported data go to BASE->Preferences and set Decimals=all.
- Use the Table exporter plug-in to export a tab-separated file with Name and Original quantity columns.
- Download the exported file to your computer and open in a spreadsheet program.
- Add a new column PoolConc and use the following formula to calculate the values:
=5 * <quantity> / 1000
- Save the results as a tab-separated file and upload it to BASE.
- Use the annotation importer to update the PoolConc values.
3. Copy missing consent information from blood to case items
Due to a bug (see #511) consent information that has already been registered on blood samples with BloodSample=PreNeo has not been copied to cases that was registered at a later time. To fix this a manual crossmatch is required to find which cases are missing consent information that is available on a blood item.
- Find all cases with missing consent information. Export a list with the case names to a tab-separated file.
- Find all PreNeo blood items that has consent information. Export the blood name, Consent and ConsentDate columns to a tab-separated file.
- Use a spreadsheet program to crossmatch the case names with the blood names.
- Copy the found consent information to new columns in the list with cases.
- Save the result as a tab-separated file and upload it to BASE.
- Use the annotation importer to import values for Consent and ConsentDate to the cases.