| 12 | | * Even without creation of extra debug files, results of some combinations of assay groups can be easily checked by inspection of the correction factor plots in the overview plots (correction values to be subtracted are shown in blue, and original values in red). If centering over genes where one group contains a single assay, the correction values will totally overlap the original values for that group, leading to a flat line at zero for that assay! Of more practical interest, grouping simultaneous-looking assays together in a group, will lead to normalized values more closely adhering to the zero line for those assays. |
| | 12 | * Even without creation of extra debug files, results of some combinations of assay groups can be easily checked by inspection of the correction factor plots in the overview plots (correction values to be subtracted are shown in blue, and original values in red). If centering over genes where one group contains a single assay, the correction values will totally overlap the original values for that assay, leading to a flat line at zero after normalization! Of more practical interest, grouping simultaneous-looking assays together in a group, will lead to normalized values more closely adhering to the zero line for those assays. |
