Changes between Initial Version and Version 23 of Ticket #764


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Timestamp:
May 12, 2015, 8:14:00 AM (7 years ago)
Author:
Nicklas Nordborg
Comment:

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  • Ticket #764

    • Property Status changed from new to assigned
    • Property Milestone changed from Reggie v3.x to Reggie v3.4
  • Ticket #764 – Description

    initial v23  
    1 With the neoprep we will be able to go directly from RNA to libraries. We'll still have to register mRNA items to indicate what is going to be processed in a batch (typically 16 RNA).
     1With the neoprep we will be able to go directly from RNA to libraries. ~~We'll still have to register mRNA items to indicate what is going to be processed in a batch (typically 16 RNA).~~
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    3 We need to select barcodes in this step and specify a target molarity and fragment size for the final libraries.
     3We need to select barcodes in this step ~~and specify a target molarity and fragment size for the final libraries~~.
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    5 Registration go directly to Libraries from the mRNA (skipping cDNA). No Caliper or Qubit files will be available. The information entered in the first step should be used instead.
     5Registration go directly to Libraries (skipping mRNA and cDNA). No Caliper or Qubit files will be available. ~~The information entered in the first step should be used instead.~~ Information about library size, molarity and volume is entered in the registration and other values (concentration and quantity) are calculated.
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    77We might have to create a new set of wizards to handle the neoprep path since it might be tricky to modify the existing wizard so they work with both the manual protocol and the neoprep.