Changes between Version 7 and Version 8 of Ticket #762
- Timestamp:
- Apr 22, 2015, 1:02:20 PM (10 years ago)
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Ticket #762 – Description
v7 v8 9 9 Due to the much lower quantity of RNA for !NeoPrep the pre-normalization will need to support mixing a larger volume (to avoid volumes less than 1µl). This also means that the mRNA wizard need to be aware of this, since it currently assumes that everything is used from the pre-normalized aliquot. 10 10 11 Another issue with the mRNA wizard is that if there are existing pre-normalized RNA for the !NeoPrep and the mRNA wizard is used with the intention of creating a manual !TruSeq plate, the !NeoPrep samples will be auto-selected and then flagged with !NotEnoughRemainingQuantity. This makes it very difficult the various protocols unless all queues are processed so they are empty before filling with RNA for a different protocol. Clearly the current auto-select function in mRNA wizard need to be much smarter about what to auto-select. The wizard also need to know about the various library preparation protocols. A separate ticket has been created for solving this issue (#773).11 Another issue with the mRNA wizard is that if there are existing pre-normalized RNA for the !NeoPrep and the mRNA wizard is used with the intention of creating a manual !TruSeq plate, the !NeoPrep samples will be auto-selected ~~and then flagged with !NotEnoughRemainingQuantity~~ (correction, they will not be flagged, but included on the mRNA plate and the lab protocol will display a very large volume in the RNA column). This makes it very difficult the various protocols unless all queues are processed so they are empty before filling with RNA for a different protocol. Clearly the current auto-select function in mRNA wizard need to be much smarter about what to auto-select. The wizard also need to know about the various library preparation protocols. A separate ticket has been created for solving this issue (#773).
