Changes between Version 6 and Version 7 of Ticket #725, comment 29


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Timestamp:
Mar 6, 2015, 3:19:40 PM (10 years ago)
Author:
olle

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  • Ticket #725, comment 29

    v6 v7  
    3131 * Step 1 should display a list of unprocessed specimen and input RNA/DNA items, from which the user can select up to 24 items.
    3232 * Step 2 should allow input of the following data:[[BR]]a. Extraction date.[[BR]] b. Extraction protocol.[[BR]]c. RNA default volume (µl).[[BR]]d. DNA default volume.[[BR]]e. QIAcube date.[[BR]]f. Primary QIAcube prefix.[[BR]]g. Secondary QIAcube prefix.[[BR]]h. QIAcube run number.[[BR]]i. Operator.[[BR]]j. Allprep DNA/RNA FFPE kit lot no.
    33  * Step 3 should display a table with 11 columns, 6 of which contain values, while the 5 other are "dummy" columns, which will get values in the next wizard. The dummy columns are shown, in order to for the two wizards to have a similar layout, and while some are present in the lab tracking sheet to be created:[[BR]]1. Specimen/RNA/DNA name for selected items.[[BR]]2. QIAcube position.[[BR]]3. Storage box for selected item, if existing.[[BR]]4. !NanoDrop plate position.[[BR]]5. RNA volume (µl).[[BR]]6. RNA concentration (ng/µl) ''(dummy)''.[[BR]]7. RNA quantity (µg) ''(dummy)''.[[BR]]8. DNA volume (µl).[[BR]]9. DNA concentration (ng/µl) ''(dummy)''.[[BR]]9. DNA quantity (µg) ''(dummy)''.[[BR]]10. DNA qPCR ΔCt value ''(dummy)''.[[BR]]11. Comment.[[BR]][[BR]]The following functionality should exist through buttons:[[BR]]a. Downloading of a !NanoDrop Sample ID file, intended to be imported in the !NanoDrop software before analysis.[[BR]]b. Downloading of a qPCR Sample ID file, intended to be imported in the qPCR software before analysis.[[BR]]c. Creation of a Lab Tracking Sheet for Allprep isolation.
     33 * Step 3 should display a table with 11 columns, 6 of which contain values, while the other 5 are "dummy" columns, which will get values in the next wizard. The dummy columns are shown, in order to for the two wizards to have a similar layout, and while some are present in the lab tracking sheet to be created:[[BR]]1. Specimen/RNA/DNA name for selected items.[[BR]]2. QIAcube position.[[BR]]3. Storage box for selected item, if existing.[[BR]]4. !NanoDrop plate position.[[BR]]5. RNA volume (µl).[[BR]]6. RNA concentration (ng/µl) ''(dummy)''.[[BR]]7. RNA quantity (µg) ''(dummy)''.[[BR]]8. DNA volume (µl).[[BR]]9. DNA concentration (ng/µl) ''(dummy)''.[[BR]]9. DNA quantity (µg) ''(dummy)''.[[BR]]10. DNA qPCR ΔCt value ''(dummy)''.[[BR]]11. Comment.[[BR]][[BR]]The following functionality should exist through buttons:[[BR]]a. Downloading of a !NanoDrop Sample ID file, intended to be imported in the !NanoDrop software before analysis.[[BR]]b. Downloading of a qPCR Sample ID file, intended to be imported in the qPCR software before analysis.[[BR]]c. Creation of a Lab Tracking Sheet for Allprep isolation.
    3434
    3535DNA/RNA registration/quantification
    … …  
    3838 * Step 1 should display a list of unprocessed specimen and input RNA/DNA items, from which the user can select up to 24 items.
    3939 * Step 2 should allow input of the following data:[[BR]]a. Extraction date.[[BR]] b. Extraction protocol.[[BR]]c. RNA default volume (µl).[[BR]]d. DNA default volume.[[BR]]e. Specimen Xylene lot no.[[BR]]f. Specimen ETOH 99.5% lot no.[[BR]]g. Specimen PKD buffer lot no.[[BR]]h. Specimen ATL buffer lot no.[[BR]]i. Specimen Proteinase K lot no.[[BR]]j. Specimen RNase A lot no.[[BR]]k. QIAcube date.[[BR]]l. Primary QIAcube prefix.[[BR]]m. Secondary QIAcube prefix.[[BR]]n. QIAcube run number.[[BR]]o. Operator.[[BR]]p. Allprep DNA/RNA FFPE kit lot no.[[BR]]q. ETOF 99.5% lot no.[[BR]]r. Buffer RLT lot no.[[BR]]s. Buffer FRN lot no.[[BR]]t. Buffer AL lot no.[[BR]]u. Buffer AW1 lot no.[[BR]]v. Buffer AW2 lot no.[[BR]]w. Buffer ATE lot no.[[BR]]x. Buffer RDD lot no.[[BR]]y. RNase free water lot no.[[BR]]z. DNAse mix lot no.[[BR]]z2. RNeasy Min Elute Spin Column.[[BR]]z3. QIAamp Min Elute Spin Column.[[BR]][[BR]]The following functionality should exist:[[BR]]a. The lot no. input fields should be pre-filled with values from the latest created DNA specimen, extracted from a specimen.[[BR]]b. It should be possible to select a text file in comma-separated format with used lot no. values, that should be imported to the input fields.
    40  * Step 3 should display a table with 11 columns, 6 of which contain values, while the 5 other are "dummy" columns, which will get values in the next wizard. The dummy columns are shown, in order to for the two wizards to have a similar layout, and while some are present in the lab tracking sheet to be created:[[BR]]1. Specimen/RNA/DNA name for selected items.[[BR]]2. QIAcube position.[[BR]]3. Storage box for selected item, if existing.[[BR]]4. !NanoDrop plate position.[[BR]]5. RNA volume (µl).[[BR]]6. RNA concentration (ng/µl) ''(dummy)''.[[BR]]7. RNA quantity (µg) ''(dummy)''.[[BR]]8. DNA volume (µl).[[BR]]9. DNA concentration (ng/µl) ''(dummy)''.[[BR]]9. DNA quantity (µg) ''(dummy)''.[[BR]]10. DNA qPCR ΔCt value ''(dummy)''.[[BR]]11. Comment.[[BR]][[BR]]The following functionality should exist through buttons:[[BR]]a. Downloading of a !NanoDrop Sample ID file, intended to be imported in the !NanoDrop software before analysis.[[BR]]b. Downloading of a qPCR Sample ID file, intended to be imported in the qPCR software before analysis.[[BR]]c. Creation of a Lab Tracking Sheet for Allprep isolation.
     40 * Step 3 should display a table with 11 columns, 6 of which contain values, while the other 5 are filled with values after the user has finished input in this wizard step:[[BR]]1. Specimen/RNA/DNA name for selected items.[[BR]]2. QIAcube position.[[BR]]3. Storage box for selected item, if existing.[[BR]]4. !NanoDrop plate position.[[BR]]5. RNA volume (µl).[[BR]]6. RNA concentration (ng/µl).[[BR]]7. RNA quantity (µg).[[BR]]8. DNA volume (µl).[[BR]]9. DNA concentration (ng/µl).[[BR]]9. DNA quantity (µg).[[BR]]10. DNA qPCR ΔCt value.[[BR]]11. Comment.[[BR]][[BR]]The following functionality should exist through buttons:[[BR]]a. It should be possible to select a !NanoDrop value file, that should be imported to give RNA concentrations.[[BR]]b. It should be possible to select a Qubit value file, that should be imported to give DNA concentrations.[[BR]]c. It should be possible to select a qPCR value file, that should be imported to give DNA qPCR ΔCt values.[[BR]]d. Creation of a Lab Tracking Sheet for Allprep isolation, with input values present in the table.[[BR]]e. Registration of newly created RNA/DNA items and updating of input RNA/DNA items with the found quantities.