Changes between Version 4 and Version 5 of Ticket #725, comment 29


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Timestamp:
Mar 6, 2015, 2:46:04 PM (10 years ago)
Author:
olle

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  • Ticket #725, comment 29

    v4 v5  
    2121 * The table section should contain the following columns:[[BR]]1. Sample ''(pre-filled)''[[BR]]2. Pos. in QIAcube ''(pre-filled)''[[BR]]3. !NanoDrop Plate Pos. ''(pre-filled)''[[BR]]4. RNA conc. ND (ng/ul)[[BR]]5. DNA conc. Qubit (ng/ul)[[BR]]6. Delta Ct[[BR]]7. Remark
    2222 * The lower info section should contain:[[BR]]1. DNA elution volume ''(pre-filled)''[[BR]]2. DNAse treated RNA[[BR]]3. Proteinase K treatment
     23
     24Two wizards should be developed:
     25 1. Lab tracking protocol for Allprep isolation
     26 2. DNA/RNA registration/quantification
     27
     28Lab tracking protocol for Allprep isolation wizard
     29 * The wizard should contain 3 steps.
     30 * Step 1 should display a list of unprocessed specimen and input RNA/DNA items, from which the user can select up to 24 items.
     31 * Step 2 should allow input of the following data:[[BR]]a. Extraction date.[[BR]] b. Extraction protocol.[[BR]]c. RNA default volume (µl).[[BR]]d. DNA default volume.[[BR]]e. QIAcube date.[[BR]]f. Primary QIAcube prefix.[[BR]]g. Secondary QIAcube prefix.[[BR]]h. QIAcube run number.[[BR]]i. Operator.[[BR]]j. Allprep DNA/RNA FFPE kit lot no.
     32 * Step 3 should display a table with 11 columns, 6 of which contain values, while the 5 other are "dummy" columns, which will get values in the next wizard. The dummy columns are shown, in order to for the two wizards to have a similar layout, and while some are present in the lab tracking sheet to be created:[[BR]]1. Specimen/RNA/DNA name for selected items.[[BR]]2. QIAcube position.[[BR]]3. Storage box for selected item, if existing.[[BR]]4. !NanoDrop plate position.[[BR]]5. RNA volume (µl).[[BR]]6. RNA concentration (ng/µl) ''(dummy)''.[[BR]]7. RNA quantity (µg) ''(dummy)''.[[BR]]8. DNA volume (µl).[[BR]]9. DNA concentration (ng/µl) ''(dummy)''.[[BR]]9. DNA quantity (µg) ''(dummy)''.[[BR]]10. DNA qPCR ΔCt value ''(dummy)''.[[BR]]11. Comment.[[BR]][[BR]]The following functionality should exist through buttons:[[BR]]a. Downloading of a !NanoDrop Sample ID file, intended to be imported in the !NanoDrop software before analysis.[[BR]]b. Downloading of a qPCR Sample ID file, intended to be imported in the qPCR software before analysis.[[BR]]c. Creation of a Lab Tracking Sheet for Allprep isolation.