Changes between Version 3 and Version 4 of Ticket #725, comment 29


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Timestamp:
Mar 6, 2015, 9:48:07 AM (9 years ago)
Author:
olle

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  • Ticket #725, comment 29

    v3 v4  
    1010
    1111More specifications concerning QIAcube runs:
    12  * Two QIAcube instruments are used, called "C" and "A":[[BR]]a. For 1-12 specimens, instrument "C" is used.[[BR]]b. For 13-24 specimens, the samples are distributed as evenly as possible between the two instruments.[[BR]]c. For each QIAcube, the samples are allocated positions according to the QIAcube placement scheme, in order to ensure stability of the centrifuge.[[BR]]d. Originally, 1 and 11 samples are not allowed in a QIAcube run, since they cannot be distributed so as to result in acceptable stability of the centrifuge. However, they will be allowed here, if a counterbalance blank vial filled with water is added, to ensure stability. A reminder note should be displayed to minimize the risk, that the counterbalance vial is forgotten.[[BR]]e. A generalized QIAcube position will be used in the form, consisting of a QIAcube instrument prefix letter "C" or "A" followed by the traditional position number in the instrument.[[BR]]f. The generalized QIAcube position will be stored as two annotations for an extract; the original QIAcube position number and a QIAcube instrument prefix.
     12 * Two QIAcube instruments are used, a primary and a secondary one. If only one instrument is needed, the primary is used. The instruments are specified by a prefix consisting of an uppercase letter. It should be possible to modify the prefixes for the primary and secondary QIAcubes, since different physical instruments might be used. The default prefixes for the primary and secondary instruments are "C" and "A", respectively:[[BR]]a. For 1-12 specimens, the primary instrument is used.[[BR]]b. For 13-24 specimens, the samples are distributed as evenly as possible between the two instruments.[[BR]]c. For each QIAcube, the samples are allocated positions according to the QIAcube placement scheme, in order to ensure stability of the centrifuge.[[BR]]d. Originally, 1 and 11 samples are not allowed in a QIAcube run, since they cannot be distributed so as to result in acceptable stability of the centrifuge. However, they will be allowed here, if a counterbalance blank vial filled with water is added, to ensure stability. A reminder note should be displayed to minimize the risk, that the counterbalance vial is forgotten.[[BR]]e. A generalized QIAcube position will be used in the form, consisting of a QIAcube instrument prefix letter followed by the traditional position number in the instrument.[[BR]]f. The generalized QIAcube position will be stored as two annotations for an extract; the original QIAcube position number and a QIAcube instrument prefix.
    1313
    1414More specifications concerning allocation of !NanoDrop plate positions:
    15  * !NanoDrop plate positions should be allocated in consecutive order, starting with "A1":[[BR]]a. Plate positions are first allocated for RNA extracted from specimens, in the order of the generalized QIAcube positions, where prefix "C" comes before "A". Note that there may be gaps in the QIAcube positions for an instrument, due to the necessity of placing the samples in a way that ensures balance of the centrifuge, but there should not be a corresponding gap in the sequence of !NanoDrop plate positions.[[BR]]b. Plate positions for input RNA are allocated in consecutive order, starting with the first available, efter positions have been allocated for newly created RNA.
     15 * !NanoDrop plate positions should be allocated in consecutive order, starting with "A1":[[BR]]a. Plate positions are first allocated for RNA extracted from specimens, in the order of the generalized QIAcube positions, where positions in the primary instrument comes before those in the secondary. Note that there may be gaps in the QIAcube positions for an instrument, due to the necessity of placing the samples in a way that ensures balance of the centrifuge, but there should not be a corresponding gap in the sequence of !NanoDrop plate positions.[[BR]]b. Plate positions for input RNA are allocated in consecutive order, starting with the first available, efter positions have been allocated for newly created RNA.
    1616
    1717More specifications concerning the created Lab Tracking Sheet for !AllPrep isolation: