Changes between Version 2 and Version 3 of Ticket #725, comment 29


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Timestamp:
Feb 27, 2015, 4:40:39 PM (9 years ago)
Author:
olle

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  • Ticket #725, comment 29

    v2 v3  
    1414More specifications concerning allocation of !NanoDrop plate positions:
    1515 * !NanoDrop plate positions should be allocated in consecutive order, starting with "A1":[[BR]]a. Plate positions are first allocated for RNA extracted from specimens, in the order of the generalized QIAcube positions, where prefix "C" comes before "A". Note that there may be gaps in the QIAcube positions for an instrument, due to the necessity of placing the samples in a way that ensures balance of the centrifuge, but there should not be a corresponding gap in the sequence of !NanoDrop plate positions.[[BR]]b. Plate positions for input RNA are allocated in consecutive order, starting with the first available, efter positions have been allocated for newly created RNA.
     16
     17More specifications concerning the created Lab Tracking Sheet for !AllPrep isolation:
     18 * The sheet should contain the following parts, from top to bottom:[[BR]]1. A header.[[BR]]2. Upper info section.[[BR]]3. Table section.[[BR]]4. Lower info section.
     19 * The header should contain two boxes:[[BR]]1. Plate ID.[[BR]]2. Operator ''(pre-filled)''.
     20 * The upper info section should contain:[[BR]]1. Date for isolation ''(pre-filled)''[[BR]]2. FFPE DNA/RNA kit lot no. ''(pre-filled)''[[BR]]3. Kapa SYBR Fast lot. no.[[BR]]4. Quality Control Primers (QCP) RGT[[BR]]5. QUBIT DNA High Sens[[BR]]6. QUBIT DNA Broad Range
     21 * The table section should contain the following columns:[[BR]]1. Sample ''(pre-filled)''[[BR]]2. Pos. in QIAcube ''(pre-filled)''[[BR]]3. !NanoDrop Plate Pos. ''(pre-filled)''[[BR]]4. RNA conc. ND (ng/ul)[[BR]]5. DNA conc. Qubit (ng/ul)[[BR]]6. Delta Ct[[BR]]7. Remark
     22 * The lower info section should contain:[[BR]]1. DNA elution volume ''(pre-filled)''[[BR]]2. DNAse treated RNA[[BR]]3. Proteinase K treatment