Changes between Initial Version and Version 1 of Ticket #725, comment 29


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Timestamp:
Feb 27, 2015, 2:41:27 PM (9 years ago)
Author:
olle

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  • Ticket #725, comment 29

    initial v1  
    88 * DNA concentrations are measured using a Qubit instrument, both for newly created and input DNA:[[BR]]a. It should be possible to input Qubit measurement values by hand in the web form.[[BR]]b. It should be possible to import measurement values from a Qubit value file, provided that the instrument is reset before the measurement session, and that the measurements are made in the same order as in the Lab Tracking Sheet for !AllPrep isolation, since the Qubit value file identifies values by timestamp, instead of sample names.
    99 * DNA delta-Ct values are measured using a qPCR instrument, both for newly created and input DNA:[[BR]]a. A Sample id file intended for use by the qPCR instrument should be created.[[BR]]b. It should be possible to import measurement values from a qPCR value file.
     10
     11More specifications concerning QIAcube runs:
     12 * Two QIAcube instruments are used, called "C" and "A":[[BR]]a. For 1-12 specimens, instrument "C" is used.[[BR]]b. For 13-24 specimens, the samples are distributed as evenly as possible between the two instruments.[[BR]]c. For each QIAcube, the samples are allocated positions according to the QIAcube placement scheme, in order to ensure stability of the centrifuge.[[BR]]d. Originally, 1 and 11 samples are not allowed in a QIAcube run, since they cannot be distributed so as to result in acceptable stability of the centrifuge. However, they will be allowed here, if a counterbalance blank vial filled with water is added, to ensure stability. A reminder note should be displayed to minimize the risk, that the counterbalance vial is forgotten.[[BR]]e. A generalized QIAcube position will be used in the form, consisting of a QIAcube instrument prefix letter "C" or "A" followed by the traditional position number in the instrument.[[BR]]f. The generalized QIAcube position will be stored as two annotations for an extract; the original QIAcube position number and a QIAcube instrument prefix.