Opened 10 years ago
Last modified 9 years ago
#672 closed task
RNA dilution wizard — at Initial Version
| Reported by: | Nicklas Nordborg | Owned by: | Nicklas Nordborg |
|---|---|---|---|
| Priority: | critical | Milestone: | Reggie v2.18 |
| Component: | net.sf.basedb.reggie | Keywords: | |
| Cc: |
Description
A new step that is performed immediately after RNA extraction and Nanodrop concentration measurements.
Instead of putting the extracts RNA in the freezer as is, an aliquot is taken and diluted to the concentration that we need in the current mRNA step. The remaining RNA is put in the freezer while the aliquot is stored temporarily since it should go into library preparation very soon.
In theory, if only such pre-diluted aliquots were used in the mRNA wizard the lab protocol would have only zeroes in the H20 column. The RNA dilution registration would not have to be used.
Also note that the mRNA wizard require that RNAQC items exists and have either RQS or RIN values. In a "real-time" scenario this will not be the case, so the wizard must work without those values.
