Opened 12 years ago
Last modified 12 years ago
#472 closed task
Export data to files needed for library preparation — at Version 5
Reported by: | Nicklas Nordborg | Owned by: | Nicklas Nordborg |
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Priority: | major | Milestone: | Reggie v2.12 |
Component: | net.sf.basedb.reggie | Keywords: | |
Cc: |
Description (last modified by )
Caliper and Qubit are used for quality control and measuring concentrations, fragment lengths, etc. Since we need to import data as annotations later, we'll probably want some files to feed the other software.
The following files are needed:
- Sample name CSV for Caliper
- Run parameters for Caliper
- Sample names for Qubit (manually edited)
Files for the Caliper are probably similar to what is produced for RNAQC (see #365, #366 and #370). Settings are different and we need two variants. Once variant when 96-well plates are used and one variant when 384-well plates are used. In the latter case coordinates should be re-mapped to only use every other well (eg. 1, 3, 5, A, C, E, etc).
Since the Qubit doesn't have much support for files, the file generated for Qubit is a template that is manually filled in and uploaded to BASE after all extracts have been processed.
Change History (5)
comment:1 by , 12 years ago
Status: | new → assigned |
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comment:2 by , 12 years ago
comment:3 by , 12 years ago
(In [1900]) References #472: Export data to files needed for library preparation
Added an export plug-in that generate a tab-separated file for the Qubit. The operator should add concentration measurements to this file and then upload to BASE (upload not yet implemented).
Added support for remapping 96 to 384 wells when exporting files for Caliper. However, the importer doesn't support this yet.
Changed default values for the CaliperLibPreprParametersExporter
to what is currently used in the lab. Still need correct plate name for 384 plates.
comment:4 by , 12 years ago
comment:5 by , 12 years ago
Description: | modified (diff) |
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(In [1896]) References #472: Export data to files needed for library preparation
Created new plug-in
CaliperLibPrepParametersExporter
. It is very similar to the existingCaliperRunParametersExporter
, except that it should have different default value (todo) and doesn't use theHiSense
option for filtering on extracts to include.