Changes between Version 3 and Version 4 of net.sf.basedb.reggie/notes34


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Timestamp:
Jun 9, 2015, 3:39:38 PM (9 years ago)
Author:
Nicklas Nordborg
Comment:

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  • net.sf.basedb.reggie/notes34

    v3 v4  
    77Ticket #764 adds support for !NeoPrep and manual !TruSeq. Since this uses less RNA than the dUTP protocol dilution protocol must support "''large mixes''" (to avoid going below the 1µl limit). This makes the dilution calculations for the mRNA dilution protocol more complex and also incompatible with mRNA plates created be earlier Reggie versions.
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    9 == 2. Create a least one Normalization protocol ==
     9== 2. Create at least one Normalization protocol ==
    1010
    1111The 'RNA concentration normalization' and 'Library preparation design' wizards need at least one protocol with subtype 'Normalization'. The protocol must specify values for the 'RNATargetVolume' (µl) and 'RNATargetAmount' (µg) annotations. It is recommended that normalization protocols are created for the procedures that are going to be used in the lab in the future. For example: