Changes between Initial Version and Version 1 of Ticket #1325


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Timestamp:
Sep 10, 2021, 2:05:23 PM (2 years ago)
Author:
Nicklas Nordborg
Comment:

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  • Ticket #1325 – Description

    initial v1  
    33It seems like the bug is only present for fragments that are shorter than the read length. It can be seen a sharp edge in the distribution of TLEN values (>0). See the attached image.
    44
     5[[Image(fragment-size-abs-tlen-hisat2.2.png)]]
     6
    57The solution is to let the `singlecolumnaverager.awk` consider both positive and negative TLEN values and then use the absolute value in the calculations. The final average and standard deviation should be the same, but the number of fragments need to be divided by 2.