Opened 5 years ago

Last modified 5 years ago

#1129 closed task

DNA pre-normalization — at Version 4

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: major Milestone: Reggie v4.23
Component: net.sf.basedb.reggie Keywords:
Cc:

Description (last modified by Nicklas Nordborg)

We are about to start processing DNA. In preparation for this we need a pre-normalization functionality that is similar to the pre-normalization done for RNA.

  • The extraction registration wizard should be updated with functionality to mark DNA that should be pre-normalized. Flagging is not needed.
  • A 'DNA concentration normalization' wizard is needed. It should have more or less the same functionality as the RNA counterpart. Flagging is not needed. We probably need to define new protocol and annotation types to hold target amounts and volumes. The default target amount and volume is 500ng in 10µl.
  • We may also need an additional label if we decide to store the pre-normalized DNA in tubes. The other option is to store the DNA in plates which requires new functionality for creating plates and assigning positions to the pre-normalized DNA.

Change History (4)

comment:1 by Nicklas Nordborg, 5 years ago

In 5356:

References #1129: DNA pre-normalization

The extraction registration wizard has been updated with a "pre-normalize" option for DNA. If selected, it will set AutoProcessing=PreNormalizeDNA on the DNA. It will not check remaining quantity except if it is 0 (or less). In this case the DNA is flagged with NotEnoughRemainingQuantity.

comment:2 by Nicklas Nordborg, 5 years ago

In 5357:

References #1129: DNA pre-normalization

Added a "Flagged DNA" list. Not actually needed at the moment, but maybe in the future.

comment:3 by Nicklas Nordborg, 5 years ago

In 5358:

References #1129: DNA pre-normalization

Added a link to DNA normalization wizard on the front page and a counter for number of items.

comment:4 by Nicklas Nordborg, 5 years ago

Description: modified (diff)
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