| 1 |
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| 2 | Overview
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| 3 | ========
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| 4 | * RNA -> mRNA (fragmented) -> cDNA
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| 5 |
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| 6 |
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| 7 | Fragmented mRNA
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| 8 | ===============
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| 9 | * name: <scanb-id>.1.l.r.m
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| 10 | * item subtype: mRNA
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| 11 | * description: <comment>
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| 12 | * original quantity: do not track
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| 13 |
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| 14 | * Creation event:
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| 15 | - date: <>
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| 16 | - protocol: ProtocolType=mRNA preparation
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| 17 |
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| 18 | * Parent item:
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| 19 | - single RNA item as parent
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| 20 | - used quantity: 1.1µg or 1.22µg (for QC)
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| 21 |
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| 22 | * Bioplate:
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| 23 | - name: mRNA<001>
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| 24 | - bioplate type: mRNA reaction plate
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| 25 | - geometry: 8x12
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| 26 | - description: <comment>
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| 27 | - files: result files from QC measurements - TODO: define file types
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| 28 |
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| 29 | * Annotations
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| 30 | - operator: String
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| 31 | - qc values: (imported from files)
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| 32 |
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| 33 |
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| 34 | cDNA
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| 35 | ====
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| 36 | * name: <scanb-id>.1.l.r.m.c
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| 37 | * item subtype: cDNA
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| 38 | * description: <comment>
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| 39 | * original quantity: do not track
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| 40 |
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| 41 | * Creation event:
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| 42 | - date: <>
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| 43 | - protocol: ProtocolType=cDNA preparation
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| 44 |
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| 45 | * Parent item
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| 46 | - single fragmented mRNA item
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| 47 | - used quantity: all (do not track)
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| 48 |
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| 49 | * Bioplate:
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| 50 | - name: cDNA<001>
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| 51 | - bioplate type: cDNA reaction plate
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| 52 | - geometry: 8x12
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| 53 | - description: <comment>
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| 54 | - files: no
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| 55 |
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| 56 | * Annotations
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| 57 | - operator: String
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| 58 |
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| 59 |
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