Changes between Version 6 and Version 7 of net.sf.basedb.reggie/notes423


Ignore:
Timestamp:
Sep 6, 2019, 10:44:38 AM (13 months ago)
Author:
Nicklas Nordborg
Comment:

Added note about sequence order for the second barcode read

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  • net.sf.basedb.reggie/notes423

    v6 v7  
    7878|| **BarcodeSequence2** || The barcode sequence for the second index read ||
    7979
     80**NOTE! ** The sequences for the second barcode are expected to be in the order that corresponds to the !NextSeq sequencer. If the sequencing has
     81been done on a !HiSeq they sequences may need to be reverse-complemented before used in the demux. See
     82https://support.illumina.com/content/dam/illumina-support/documents/documentation/system_documentation/miseq/indexed-sequencing-overview-guide-15057455-05.pdf
     83for more information.
     84
    8085 2. A barcode template is represented as a **Bioplate** item where each coordinate is linked to a **Tag** item. Start by manually creating the required number of bioplates. They should have the following values:
    8186