Changes between Version 1 and Version 2 of net.sf.basedb.reggie/notes34


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Timestamp:
May 12, 2015, 8:09:38 AM (9 years ago)
Author:
Nicklas Nordborg
Comment:

Adding update notes related to ticket #762

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  • net.sf.basedb.reggie/notes34

    v1 v2  
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    77Ticket #764 adds support for !NeoPrep and manual !TruSeq. Since this uses a less RNA than the dUTP protocol dilution protocol must support "''large mixes''" (to avoid going below the 1µl limit). This makes the dilution calculations for the mRNA dilution protocol more complex and also incompatible with mRNA plates created be earlier Reggie versions.
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     9== 2. Create a least one Normalization protocol ==
     10
     11The 'RNA concentration normalization' and 'Library preparation design' wizards need at least one protocol with subtype 'Normalization'. The protocol must specify values for the 'RNATargetVolume' (µl) and 'RNATargetAmount' (µg) annotations. It is recommended that normalization protocols are created for the procedures that are going to be used in the lab in the future. For example:
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     13 * Manual !TruSeq, 50µl, 0.5µg
     14 * !NeoPrep !TruSeq, 45µl, 0.1µg
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     16See also ticket #762.