Opened 4 years ago

Closed 4 years ago

#873 closed enhancement (fixed)

Target volume for NeoPrep should be changed from 12.5 to 15

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: major Milestone: Reggie v4.2.1
Component: net.sf.basedb.reggie Keywords:
Cc:

Description (last modified by Nicklas Nordborg)

See also #858. Turned out that 12.5µl was not practical since it was difficult to take all without introducing air bubbles.

The new target volume should be 15µl. However in the end, only 12.5µl is going into the NeoPrep, but that is happening outside of BASE. Since not all of the 15µl is used we need to correct the amount of RNA so that the 12.5µl that is used still contains 100ng. We need 120ng in 15µl.

Note: In the future it might be better to use protocol annotations for this (similar to how the pre-normalization) works.

Change History (4)

comment:1 Changed 4 years ago by Nicklas Nordborg

(In [3805]) References #873: Target volume for NeoPrep? should be changed from 12.5 to 15

Updated the taget volume and added a section for displaying this in the wizard (to the right of the amount input boxes).

comment:2 Changed 4 years ago by Nicklas Nordborg

Description: modified (diff)

comment:3 Changed 4 years ago by Nicklas Nordborg

(In [3806]) References #873: Target volume for NeoPrep? should be changed from 12.5 to 15

Default RNA target amount for NeoPrep set to 0.12µg.

comment:4 Changed 4 years ago by Nicklas Nordborg

Resolution: fixed
Status: newclosed
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