Opened 6 years ago

Closed 6 years ago

#773 closed enhancement (fixed)

The mRNA wizard must be smarter when auto-selecting pre-normalized RNA

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: critical Milestone: Reggie v3.4
Component: net.sf.basedb.reggie Keywords:
Cc:

Description (last modified by Nicklas Nordborg)

If the 'Pre-normalized RNA' option is selected in the auto-select function, it will select all pre-normalized RNA not accounting for the intended protocol (see #762).

This means that RNA which is pre-normalized for the NeoProp will be flagged if the mRNA wizard is used to create a manual TruSeq plate. Other mismatches may create incorrect mixing protocols.

Seems like the mRNA wizard will allow all pre-normalized RNA to be included but with a warning if the quantity is too low. The lab protocol doesn't make any checks either but will show incorrect volumes.

The mRNA wizard need to be aware of the different library preparation protocols and only auto-select RNA that is for the intended use. Exactly how to do this needs to be investigated.

Change History (7)

comment:1 Changed 6 years ago by Nicklas Nordborg

Summary: The auto-select function in the mRNA wizard must be smarter when selecting pre-normalized RNAThe mRNA wizard must be smarter when auto-selecting pre-normalized RNA

comment:2 Changed 6 years ago by Nicklas Nordborg

Description: modified (diff)

comment:3 Changed 6 years ago by Nicklas Nordborg

Description: modified (diff)

comment:4 Changed 6 years ago by Nicklas Nordborg

Status: newassigned

comment:5 Changed 6 years ago by Nicklas Nordborg

(In [3270]) References #773: The mRNA wizard must be smarter when auto-selecting pre-normalized RNA

Added selection of normalization protocol in the auto-select RNA function so that it only selects RNA that has been pre-normalized with the given protocol.

comment:6 Changed 6 years ago by Nicklas Nordborg

(In [3272]) References #773: The mRNA wizard must be smarter when auto-selecting pre-normalized RNA

Not enough remaining quantity is now an error condition instead of a warning. If it still manages to get through to the lab protocol a warning is displayed there as well.

comment:7 Changed 6 years ago by Nicklas Nordborg

Resolution: fixed
Status: assignedclosed
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