Opened 7 years ago

Closed 7 years ago

#555 closed enhancement (fixed)

Specify amount to use from *.dil items in pool

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: minor Milestone: Reggie v2.14
Component: net.sf.basedb.reggie Keywords:
Cc:

Description

When dynamic mixing strategy is used for a pool, libraries which need less than 1µl in the pool are mixed separately before they are added to the pool. The current strategy is to mix the library to 2nM and then add the specified 'Average volume' (5µl) to the pool.

In most cases this is good enough, but if there are also many libraries with low concentration it may happen that we end up with a negative EB volume to add in the end. In this case it may be useful to mix less EB with the high-concentration libraries then add less to the pool (5nM and 2µl or 10nM and 1µl) so that the final EB volume increases (hopefully to a value > 0).

Change History (4)

comment:1 Changed 7 years ago by Nicklas Nordborg

Status: newassigned

comment:2 Changed 7 years ago by Nicklas Nordborg

(In [2186]) References #555: Specify amount to use from *.dil items in pool

The volume to use in the pool can now be set by selecting the wells and clicking the 'Separate mix volume' button.

As a side-effect the mix factor is now calculated dynamically so that around 2µl is used from the original lib. The previous implementation with a fixed mix factor could still yield lower than 1µl for libraries with very high contentration (>20nM).

comment:3 Changed 7 years ago by Nicklas Nordborg

(In [2188]) References #555: Specify amount to use from *.dil items in pool

Implemented the same functionality in the "Create manual pool" wizard. Also discovered and fixed some bugs introduced by #553 (submitting mixingStrategy and target volume as properties of the pool).

comment:4 Changed 7 years ago by Nicklas Nordborg

Resolution: fixed
Status: assignedclosed
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