Changes between Version 4 and Version 5 of Ticket #533


Ignore:
Timestamp:
Dec 10, 2013, 10:59:58 AM (10 years ago)
Author:
Nicklas Nordborg
Comment:

Legend:

Unmodified
Added
Removed
Modified

  • Ticket #533 – Description

    v4 v5  
    99 2. (#546) Confirm sequencing as completed. First wizard in the "Secondary analysis wizards" section. Used to decide if the sequenced data is ok or not. If ok, continue with demuxing, otherwise flag pools for re-sequencing.
    1010
    11  3. Start demux and merge. This wizard is starts the demux and merge operations.
     11 3. (#547) Start demux and merge. This wizard starts the demux and merge operations.
    1212
    1313 4. Register demux and merge as ended. At the end we have one "!MergedSequences" item for each "Library" from the flow cells that was sequenced. A count of the number of reads for each library must be recorded and is used to determine if the library needs to be re-sequenced or not. FASTQ files for each library are stored on the server.
    1414
    15  5. Start filtering and alignment. Bowtie and TopHat is used to first filter and then align against a pre-defined set of transcripts.
     15 5. Start filtering and alignment. Bowtie and !TopHat is used to first filter and then align against a pre-defined set of transcripts.
    1616
    1717 6. Register filtering and alignment as ended. At the end we have one "!AlignedSequences" item for each "Library" from the flow cells that was sequenced. BAM files for each library are stored on the server.