Opened 11 years ago
Closed 11 years ago
#504 closed enhancement (fixed)
Allow target volume/library in a pool to be changed
| Reported by: | Nicklas Nordborg | Owned by: | Nicklas Nordborg |
|---|---|---|---|
| Priority: | major | Milestone: | Reggie v2.13 |
| Component: | net.sf.basedb.reggie | Keywords: | |
| Cc: |
Description
Currently, the target volume for each library in a pool is fixed at 5µl. In the normal procedure with 21 samples per pool it means a final pool volume of 105µl. Since only 10µl is needed in the next step (clustering), it would be useful to be able to have a lower target volume per library.
The limiting factor is that it is impractical to pick less than 1µl from a library, so if too many libraries have high concentration it adds extra work due to first mixing a higher volume before pooling. On the other hand, libraries with low concentration benefit from this change, since less material is needed.
Change History (3)
comment:1 by , 11 years ago
| Status: | new → assigned |
|---|
comment:2 by , 11 years ago
comment:3 by , 11 years ago
| Resolution: | → fixed |
|---|---|
| Status: | assigned → closed |
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(In [2025]) References #504: Allow target volume/library in a pool to be changed
This should now be implemented, but since #503 will change how the calculations are done again, the final fine-tuning will be made as part of that ticket instead.