Opened 6 years ago

Closed 6 years ago

#504 closed enhancement (fixed)

Allow target volume/library in a pool to be changed

Reported by: Nicklas Nordborg Owned by: Nicklas Nordborg
Priority: major Milestone: Reggie v2.13
Component: net.sf.basedb.reggie Keywords:
Cc:

Description

Currently, the target volume for each library in a pool is fixed at 5µl. In the normal procedure with 21 samples per pool it means a final pool volume of 105µl. Since only 10µl is needed in the next step (clustering), it would be useful to be able to have a lower target volume per library.

The limiting factor is that it is impractical to pick less than 1µl from a library, so if too many libraries have high concentration it adds extra work due to first mixing a higher volume before pooling. On the other hand, libraries with low concentration benefit from this change, since less material is needed.

Change History (3)

comment:1 Changed 6 years ago by Nicklas Nordborg

Status: newassigned

comment:2 Changed 6 years ago by Nicklas Nordborg

(In [2025]) References #504: Allow target volume/library in a pool to be changed

This should now be implemented, but since #503 will change how the calculations are done again, the final fine-tuning will be made as part of that ticket instead.

comment:3 Changed 6 years ago by Nicklas Nordborg

Resolution: fixed
Status: assignedclosed

Fixed in [2027] as part of #503.

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